Extraction of streptococcal type 12 M protein by cyanogen bromide
نویسندگان
چکیده
منابع مشابه
Vimentin-cross-reactive epitope of type 12 streptococcal M protein.
The NH2-terminal amino acid sequence of type 12 M protein was determined by automated Edman degradation of a 38-kilodalton polypeptide fragment purified from a limited pepsin digest of intact type 12 streptococci. The sequence of the first 13 amino acid residues of the polypeptide confirmed that predicted by the nucleotide sequence of the mature type 12 M protein. A chemically synthesized pepti...
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Streptococcal M proteins are antiphagocytic molecules varying immunologically from type to type while maintaining an identical biological effect. The virulence of the group A streptococcus is directly related to the presence of the M antigens on the cell surface and resistance to infection by these organisms is dependent on the presence of opsonic antibodies directed towards the M molecules. Va...
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Evidence confirming the identity of fibrinogen-precipitating factor and streptococcal M protein is provided by the demonstration of bactericidal, mouse protective, and long chain-producing antibodies in the sera of rabbits immunized with washed M-fibrinogen precipitates. Two precipitin lines were observed in immunoelectrophoresis of human plasma vs. rabbit anti-M-fibrinogen antiserum; no precip...
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M proteins that define the serotypes of group A streptococci are powerful blastogens for human T lymphocytes. The mechanism by which they activate T cells was investigated and compared with the conventional T cell mitogen phytohemagglutinin, and the known superantigen staphylococcal enterotoxin B. Although major histocompatibility complex (MHC) class II molecules are required for presentation, ...
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Optimum conditions for the assay of thiamin were studied using a cyanogen bromide (BrCN) oxidation method. The adopted procedure included neither pre-purification of samples through an ion exchanger nor extraction of the thiochrome into an organic solvent. The 0.25 M BrCN (the concentration before the addition of alkali) and the final NaOH concentration of approx. 1% gave the highest yield of t...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1978
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.21.2.546-555.1978